Evaluation of F1 coffee hybrids (Coffea arabica L.) and their respective parents with RAPD markers
F1 coffee (C. arabica) hybrids and their parents were evaluated using random amplified polymorphic DNA (RAPD) markers. The population used in this study was from the coffee breeding programme of the Plant Pathology Department of the Federal University of Vicosa (Minas Gerais, Brazil), descendant of crossings between ancestries of Catuai with red and yellow berries and Timor Hybrid selections. To study the genetic diversity, 51 genotypes (10 Catuai ancestors, 12 Timor Hybrid ancestors and 29 F1 hybrids) were used. In the genetic diversity study, a total of 157 reactions, with 86 primers were carried out, with 53.5 percent of them presenting polymorphic bands (a total of 108 with an average of 2.35 polymorphic bands/primer). The primers showing the greatest number of polymorphic bands were OPA-8, OPC-10, OPA-5 and OPA-10. Limited to a genetic distance of 54 percent, Timor Hybrid UFV 427-15 remained in the group of Catuai. Eleven of the twelve more productive genotypes in the F1 generation confirmed their hybrid nature. It is concluded that RAPD markers were efficient in evaluating the genetic diversity in C. arabica and in certifying the hybrid condition of the genetic materials obtained by artificial crosses.
| Main Authors: | , , , , |
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| Format: | biblioteca |
| Published: |
2002
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| Subjects: | COFFEA, COFFEA ARABICA, DISTANCIA GENETICA, VARIACION GENETICA, MARCADORES GENETICOS, HIBRIDOS, FITOMEJORAMIENTO, RAPD, |
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| Summary: | F1 coffee (C. arabica) hybrids and their parents were evaluated using random amplified polymorphic DNA (RAPD) markers. The population used in this study was from the coffee breeding programme of the Plant Pathology Department of the Federal University of Vicosa (Minas Gerais, Brazil), descendant of crossings between ancestries of Catuai with red and yellow berries and Timor Hybrid selections. To study the genetic diversity, 51 genotypes (10 Catuai ancestors, 12 Timor Hybrid ancestors and 29 F1 hybrids) were used. In the genetic diversity study, a total of 157 reactions, with 86 primers were carried out, with 53.5 percent of them presenting polymorphic bands (a total of 108 with an average of 2.35 polymorphic bands/primer). The primers showing the greatest number of polymorphic bands were OPA-8, OPC-10, OPA-5 and OPA-10. Limited to a genetic distance of 54 percent, Timor Hybrid UFV 427-15 remained in the group of Catuai. Eleven of the twelve more productive genotypes in the F1 generation confirmed their hybrid nature. It is concluded that RAPD markers were efficient in evaluating the genetic diversity in C. arabica and in certifying the hybrid condition of the genetic materials obtained by artificial crosses. |
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