Genetic variability of Beauveria bassiana associated with the coffee berry borer Hypothenemus hampei and other insects
Genetic variability of 95 isolates of B. bassiana from different geographical regions and hosts was assessed using RAPDs and ITS-RFLP molecular markers to characterize its genetic variability. Clusterings were obtained using UPGMA and Principal Coordinates Analysis. Four groups were identified with RAPDs. A 930 bp fragment was amplified with primers ITS1 and PN16, and polymorphisms were observed with AluI and MspI, generating two main clusters. Results indicate low genetic variability present in theColombian B. bassiana population, no association to host type or geographic locality, and a population structure that is not clonal. This characterization sets criteria for determination of bio-insecticide potentiality, introduction of foreign strains and improvement of entomopathogens within an IPM programme.
| Main Authors: | , , , , |
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| Format: | biblioteca |
| Published: |
2002
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| Subjects: | COFFEA, HYPOTHENEMUS HAMPEI, BEAUVERIA BASSIANA, INSECTOS DAÑINOS, PLAGAS DE PLANTAS, AGENTES DE CONTROL BIOLOGICO, MARCADORES GENETICOS, POLIMORFISMO GENETICO, VARIACION GENETICA, SECUENCIA NUCLEOTIDICA, |
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| Summary: | Genetic variability of 95 isolates of B. bassiana from different geographical regions and hosts was assessed using RAPDs and ITS-RFLP molecular markers to characterize its genetic variability. Clusterings were obtained using UPGMA and Principal Coordinates Analysis. Four groups were identified with RAPDs. A 930 bp fragment was amplified with primers ITS1 and PN16, and polymorphisms were observed with AluI and MspI, generating two main clusters. Results indicate low genetic variability present in theColombian B. bassiana population, no association to host type or geographic locality, and a population structure that is not clonal. This characterization sets criteria for determination of bio-insecticide potentiality, introduction of foreign strains and improvement of entomopathogens within an IPM programme. |
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