Algunos aspectos biológicos de Cercospora coffeicola
The iron spot disease attacks coffee leaves and fruits with high incidence and severity in the Colombian coffee zone, especially in crops directly exposed to solar radiation and low nutriments supuply. The difficulty to achieve isolation, growth and fungus sporulation in a synthetic media has been one of the greatest limitations to carry out the biologic studies. In order to obtain information on the matter, a collection of naturally infected leaves was made at Cenicafé (La Granja), in the Central Station Naranjal and in the experimental sub-stations in Supía (Caldas), Maracay (Quindío) y Sasaima (Cundinamarca). The samples were disinfested with sterile distillated water SDW, sodium hypochlorite or ethanol. The fungus was cultured in two synthetic media, modified juice V8 and coffee leaves extract, oaths, agar (CAA). In each isolation the average rate of radial growth, sporulation and pathogenicity conditions were measured. The isolation with SDW displayed a better answer. Seven isolations of the pathogen were codified according to their physical appearance (Cc9901 a Cc9907). The average rate of radial growth did not exhibit statistical differences (p = 0.05) for the culture media, nonetheless, with CAA media the highest sporulation was obtained. Variability among the isolations with respect to pathogenicity was observed.
| Main Authors: | , , |
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| Format: | biblioteca |
| Published: |
Jul-
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| Subjects: | COFFEA ARABICA, CERCOSPORA COFFEICOLA, ENFERMEDADES FUNGOSAS, AISLAMIENTO, CICLO VITAL, PATOGENICIDAD, |
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| Summary: | The iron spot disease attacks coffee leaves and fruits with high incidence and severity in the Colombian coffee zone, especially in crops directly exposed to solar radiation and low nutriments supuply. The difficulty to achieve isolation, growth and fungus sporulation in a synthetic media has been one of the greatest limitations to carry out the biologic studies. In order to obtain information on the matter, a collection of naturally infected leaves was made at Cenicafé (La Granja), in the Central Station Naranjal and in the experimental sub-stations in Supía (Caldas), Maracay (Quindío) y Sasaima (Cundinamarca). The samples were disinfested with sterile distillated water SDW, sodium hypochlorite or ethanol. The fungus was cultured in two synthetic media, modified juice V8 and coffee leaves extract, oaths, agar (CAA). In each isolation the average rate of radial growth, sporulation and pathogenicity conditions were measured. The isolation with SDW displayed a better answer. Seven isolations of the pathogen were codified according to their physical appearance (Cc9901 a Cc9907). The average rate of radial growth did not exhibit statistical differences (p = 0.05) for the culture media, nonetheless, with CAA media the highest sporulation was obtained. Variability among the isolations with respect to pathogenicity was observed. |
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