Multiplicacao 'in vitro' de Pratylenchus brachyurus, Pratylenchus zeae, Radopholus similis e Tylenchorhynchus sp.
Five methods were evaluated for axenizing Pratylenchus brachyurus, P. zeae, Radopholus similis and Tylenchorhynchus sp. Methods using mercuric chloride and combinations of mercuric chloride with Streptomycin sulfate or Streptomycin sulfate with Neantin were the most efficient. The effect of four culture media on the reproduction of nematodes in alfalfa callus cultures were compared. The simplified medium from Riedel et alii, which consists of yeast extract, 2,4-D, sucrose and agar led to reproduction of P. brachyurus, P. zeae and R. similis and results were comparable to complex traditional culture media. Alfalfa callus obtained from different culture media was not promising for Tylenchorhynchus sp. reproduction. The influence of two alfalfa cultivars and of a modification of the Riedel et alii medium on the reproduction of P. brachyurus, P. zeae and R. similis was studied under laboratory conditions. The alfalfa cultivars 'Moapa' and 'Crioula' presented the same efficiency when cultivated in the medium of Riedel et alii or its modification. The addition of potato decoction to the medium of Riedel et alii increased callus development in both alfalfa cultivars; nevertheless it, significantly reduced nematode reproduction. The effect of temperature on nematode reproduction in monoxenic cultures of alfalfa callus in the media of Riedel et alii was studied. Pratylenchus brachyurus and R. similis presented greater reproduction at 25-30°C and P. zeae at 25-35°C. The maximum reproduction was estimated at 28, 25 and 30°C, respectively. Multiplication techniques for P. brachyurus, P. zeae, R. similis and Tylenchorhynchus sp. using carrot disk in Petri dishes with water-agar and carrot disks in bottles (40 mm diameter x 50 mm height) without water-agar were compared. The former was more successful for nematode multiplication.
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| Format: | biblioteca |
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Vicosa, MG (Brasil)
1986
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| Subjects: | PRATYLENCHUS BRACHYURUS, PRATYLENCHUS ZEAE, RADOPHOLUS SIMILIS, TYLENCHORHYNCHUS, NEMATODOS DE LAS PLANTAS, CULTIVO IN VITRO, HISTOLOGIA, |
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| Summary: | Five methods were evaluated for axenizing Pratylenchus brachyurus, P. zeae, Radopholus similis and Tylenchorhynchus sp. Methods using mercuric chloride and combinations of mercuric chloride with Streptomycin sulfate or Streptomycin sulfate with Neantin were the most efficient. The effect of four culture media on the reproduction of nematodes in alfalfa callus cultures were compared. The simplified medium from Riedel et alii, which consists of yeast extract, 2,4-D, sucrose and agar led to reproduction of P. brachyurus, P. zeae and R. similis and results were comparable to complex traditional culture media. Alfalfa callus obtained from different culture media was not promising for Tylenchorhynchus sp. reproduction. The influence of two alfalfa cultivars and of a modification of the Riedel et alii medium on the reproduction of P. brachyurus, P. zeae and R. similis was studied under laboratory conditions. The alfalfa cultivars 'Moapa' and 'Crioula' presented the same efficiency when cultivated in the medium of Riedel et alii or its modification. The addition of potato decoction to the medium of Riedel et alii increased callus development in both alfalfa cultivars; nevertheless it, significantly reduced nematode reproduction. The effect of temperature on nematode reproduction in monoxenic cultures of alfalfa callus in the media of Riedel et alii was studied. Pratylenchus brachyurus and R. similis presented greater reproduction at 25-30°C and P. zeae at 25-35°C. The maximum reproduction was estimated at 28, 25 and 30°C, respectively. Multiplication techniques for P. brachyurus, P. zeae, R. similis and Tylenchorhynchus sp. using carrot disk in Petri dishes with water-agar and carrot disks in bottles (40 mm diameter x 50 mm height) without water-agar were compared. The former was more successful for nematode multiplication. |
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