Standardization of encapsulation technique for producing synthetic seeds in coffee
The zygotic embryos of Coffea arabica viz., S.4348 and S.2794 were encapsulated in sodium alginate gel with 0.1 mg/l of BAP in MS medium by dropping into 2 per cent calcium chloride solution resulting in the formation of round beads. These encapsulated beads with zygotic embryos when sown directly in the potted vermiculite soil, could not be regenerated into plantlets. However, fast germination and plantlet development was achieved on basal half strength MS medium and half strenght MS medium supplemented with 0.1 mg/l of BAP. The practical significance of the encapsulation techniques are discussed.
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| Main Authors: | , |
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| Format: | biblioteca |
| Published: |
1995
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| Subjects: | COFFEA ARABICA, EMBRIONES VEGETALES, CIGOTOS, SEMILLAS, PRODUCCION DE SEMILLAS, ENCAPSULACION, |
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| Summary: | The zygotic embryos of Coffea arabica viz., S.4348 and S.2794 were encapsulated in sodium alginate gel with 0.1 mg/l of BAP in MS medium by dropping into 2 per cent calcium chloride solution resulting in the formation of round beads. These encapsulated beads with zygotic embryos when sown directly in the potted vermiculite soil, could not be regenerated into plantlets. However, fast germination and plantlet development was achieved on basal half strength MS medium and half strenght MS medium supplemented with 0.1 mg/l of BAP. The practical significance of the encapsulation techniques are discussed. |
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