The differentiation of coffee plants through tissue culture: the effect of auxin/kinetin concentrations and light intensity
Callus formation in segments taken from young orthotropic shoots of mature plants of Coffea robusta was induced by culturing the explants in Linsmaier and Skoog (LS) basal medium supplemented with 1 ppm naphthalenic acetic acid (NAA) and varying concentrations of kinetin under conditions of different light intensities. Optimal callus growth was obtained with 1 ppm NAA combined with 0,1 ppm kinetin in cultures incubated in the dark. On subculturing the callus on LS medium with NAA concentration increased to 2 ppm and kinetin to 1 ppm, protocorms or organoid bodies were developed. Under continuous light at an intensity of 350 lux, the protocorm was stimulated to form coloured leaf primordia and primary roots, which further developed into plantlets upon transfer to Gresshoff-Doy basal medium (without growth regulators).
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| Format: | biblioteca |
| Published: |
1980
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| Subjects: | COFFEA CANEPHORA, CULTIVO DE TEJIDOS, CALLO, AUXINAS, QUINETINA, SUSTANCIAS DE CRECIMIENTO VEGETAL, EXPLANTES, MEDIO DE CULTIVO, LUZ DEL DIA, ACIDO NAFTILACETICO, |
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| Summary: | Callus formation in segments taken from young orthotropic shoots of mature plants of Coffea robusta was induced by culturing the explants in Linsmaier and Skoog (LS) basal medium supplemented with 1 ppm naphthalenic acetic acid (NAA) and varying concentrations of kinetin under conditions of different light intensities. Optimal callus growth was obtained with 1 ppm NAA combined with 0,1 ppm kinetin in cultures incubated in the dark. On subculturing the callus on LS medium with NAA concentration increased to 2 ppm and kinetin to 1 ppm, protocorms or organoid bodies were developed. Under continuous light at an intensity of 350 lux, the protocorm was stimulated to form coloured leaf primordia and primary roots, which further developed into plantlets upon transfer to Gresshoff-Doy basal medium (without growth regulators). |
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