The effect of ethephon and adenine on purine alkaloid synthesis in suspension-cultured cells of Coffea arabica L
The effect of ethephon, an ethylene-releasing compound, was studied in cell suspension cultures of Coffea arabica cultivated either under photoperiod or in the dark. In darkness purine alkaloid production, especially the synthesis of the caffeine precursors 7-methylxanthine and theobromine, is increased. Cells cultivated under photoperiod are very sensitive to ethephon: low concentrations stimulate alkaloid formation whereas high amounts inhibit purine alkaloid and chlorogenic acid synthesis, both usually stimulated by light. When ethephon is added in combination with adenine, a widely accepted early precursor of purine alkaloid biosynthesis, a drastic stimulation of 7-methylxanthine and theobromine synthesis can be observed in the dark.
| Main Authors: | , |
|---|---|
| Format: | biblioteca |
| Published: |
1992
|
| Subjects: | COFFEA ARABICA, CULTIVO DE CELULAS, ETEFON, ADENINA, PURINAS, ALCALOIDES, |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | The effect of ethephon, an ethylene-releasing compound, was studied in cell suspension cultures of Coffea arabica cultivated either under photoperiod or in the dark. In darkness purine alkaloid production, especially the synthesis of the caffeine precursors 7-methylxanthine and theobromine, is increased. Cells cultivated under photoperiod are very sensitive to ethephon: low concentrations stimulate alkaloid formation whereas high amounts inhibit purine alkaloid and chlorogenic acid synthesis, both usually stimulated by light. When ethephon is added in combination with adenine, a widely accepted early precursor of purine alkaloid biosynthesis, a drastic stimulation of 7-methylxanthine and theobromine synthesis can be observed in the dark. |
|---|