Sialic acid in plant tissues: a history of incorrect identification due to the use of non-specific colorimetric reactions
Finely ground seeds of wheat, sunflower, coffee and french bean were either hydrolyses in acid directly, or after extraction with ethanol and/or after treatment in alkali to hydrolyse any O-acetyl sialic acids present. The acid hydrolysates were fractionated by ion-exchange chromatography. Sialic acid was not detected in any of the materials examined but quinic acid was found in all of them. It is concluded that some previous reports of siatic acid in plant seeds and tissues may have been due to the presence of quinic acid and of other compounds, which show rather similar properties to sialic acid in chromatographic and colorimetric methods of analysis
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| Format: | biblioteca |
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| Published: |
Nov
|
| Subjects: | BIOQUIMICA, GRANOS, COMPOSICION, CROMATOGRAFIA, ANALISIS DEL GRANO, |
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| Summary: | Finely ground seeds of wheat, sunflower, coffee and french bean were either hydrolyses in acid directly, or after extraction with ethanol and/or after treatment in alkali to hydrolyse any O-acetyl sialic acids present. The acid hydrolysates were fractionated by ion-exchange chromatography. Sialic acid was not detected in any of the materials examined but quinic acid was found in all of them. It is concluded that some previous reports of siatic acid in plant seeds and tissues may have been due to the presence of quinic acid and of other compounds, which show rather similar properties to sialic acid in chromatographic and colorimetric methods of analysis |
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