Sur la formation d'un complexe depside-cafeine lors de la conservation, au froid, en milieu hydro-©♭thanolique d'echantillons foliaires de cafeiers. Implications methodologiques pour l'extraction des depsides
The depsides present in coffee leaves are not apparent upon extraction with ethanol if the plant material has been fixed in boiling ethanol then maintained at - 25ðC. A complex formed with cafein prevents the extraction and this artefact from cold conservation concerns chlorogenic acid and its isomers. A treatment with chloroform can break the complex and allows the depsides to be dosed. The complex does not seem to exist in living issues
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| Format: | biblioteca |
| Published: |
Jul
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| Subjects: | BIOQUIMICA, HOJAS, EXTRACCION, CAFEINA, ANALISIS DE TEJIDOS, ANALISIS QUIMICO, ACIDO CLOROGENICO, |
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| Summary: | The depsides present in coffee leaves are not apparent upon extraction with ethanol if the plant material has been fixed in boiling ethanol then maintained at - 25ðC. A complex formed with cafein prevents the extraction and this artefact from cold conservation concerns chlorogenic acid and its isomers. A treatment with chloroform can break the complex and allows the depsides to be dosed. The complex does not seem to exist in living issues |
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