Studies on the use of banana trees (Musa sp.) for coffee and cocoa shading, 2: Supply of in vitro seedlings, multiplication of micro shoots of Musa paradisiaca on in vitro culture
Multiplication of banana micro shoots had been studied in the Laboratory of Tissue Culture of Indonesian Coffee and Cocoa Research Institute (ICCRI). The aim of this experiment was to study the effect of Naphthaleneacetic acid (NAA), Benzylaminopurine (BAP), adenine and coconut water on the micro shoots multiplication. The experiment consisted of two stages. The first stage was aimed to find out the effect of adenine and BAP on micro shoot multiplication on the media enriched with NAA. The second stage was done after determining the optimal concentration of BAP which was aimed to find out the effect of BAP, NAA and coconut water. The result showed that BAP and coconut water had good effect. The most appropriate concentration of BAP was 1.0 mg/l. The role of BAP in this process could be replaced by 1.0 percent coconut water.
Main Authors: | , |
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Format: | biblioteca |
Published: |
Jul
|
Subjects: | THEOBROMA CACAO, COFFEA ARABICA, MUSA (BANANOS), MUSA (PLATANOS), SOMBRA, ARBOLES DE SOMBRA, PLANTULAS, CULTIVO IN VITRO, CULTIVO DE TEJIDOS, |
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Summary: | Multiplication of banana micro shoots had been studied in the Laboratory of Tissue Culture of Indonesian Coffee and Cocoa Research Institute (ICCRI). The aim of this experiment was to study the effect of Naphthaleneacetic acid (NAA), Benzylaminopurine (BAP), adenine and coconut water on the micro shoots multiplication. The experiment consisted of two stages. The first stage was aimed to find out the effect of adenine and BAP on micro shoot multiplication on the media enriched with NAA. The second stage was done after determining the optimal concentration of BAP which was aimed to find out the effect of BAP, NAA and coconut water. The result showed that BAP and coconut water had good effect. The most appropriate concentration of BAP was 1.0 mg/l. The role of BAP in this process could be replaced by 1.0 percent coconut water. |
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