Proliferation and rooting of cotyledonary nodal shoots of cacao
Cotyledonary nodes of cacao (Theobroma cacao L.) cultured in semisolid medium based on Murashige and Skoog salts supplemented with vitamins, casei, hydrolysate, and 3 per cent sucrose increased axillary shoot production in response to 4.4 micra M 6-benzylamino purine (BA) and 0.005-1.0 micra M thidiazuron after removal of the main shoot. Thidiazuron was more effective than BA in increasing the number of shoots per node. Main and axillary shoots of cacao seedlings grown in vivo rooted in a soil-perlite (1:1) medium in response to treatment of the base with IBA and/or NAA at 2000-8000 ppm in 50 per cent ethanol for 10 seconds with maximum response achieved with IBA at 8000 ppm. Detached axillary shoots derived from the cotyledonary node failed to grow or proliferate in vitro with standard media manipulation including reduced salt concentration, BA, zeatin, or thidiazuron, gelrite or agar, or cotyledonary extracts
| Main Authors: | , , |
|---|---|
| Format: | biblioteca |
| Published: |
1987
|
| Subjects: | THEOBROMA CACAO, PLANTULAS, CULTIVO DE MERISTEMOS, ENRAIZAMIENTO, COTILEDONES, |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Cotyledonary nodes of cacao (Theobroma cacao L.) cultured in semisolid medium based on Murashige and Skoog salts supplemented with vitamins, casei, hydrolysate, and 3 per cent sucrose increased axillary shoot production in response to 4.4 micra M 6-benzylamino purine (BA) and 0.005-1.0 micra M thidiazuron after removal of the main shoot. Thidiazuron was more effective than BA in increasing the number of shoots per node. Main and axillary shoots of cacao seedlings grown in vivo rooted in a soil-perlite (1:1) medium in response to treatment of the base with IBA and/or NAA at 2000-8000 ppm in 50 per cent ethanol for 10 seconds with maximum response achieved with IBA at 8000 ppm. Detached axillary shoots derived from the cotyledonary node failed to grow or proliferate in vitro with standard media manipulation including reduced salt concentration, BA, zeatin, or thidiazuron, gelrite or agar, or cotyledonary extracts |
|---|