Hormonal control of cellular proliferation in cultured callus derived from Theobroma cacao L.
Optimal cellular proliferation for cacao leaf tissue and fruit explants occurred on modified Murashige and Skoog's medium supplemented with kinetin (2-6 mg/1) in the absence of indole-3-acetic acid (IAA) or with zeatin (0.25 - 1.0 mg/1) and IAA (0.0 - 0.5 mg/1). Theobromine does not seem to be a regulatory molecule in culture explants of cacao but proved to be an effective agent in the control of phenolic oxidation. Other effective chemicals for the control of phenolic oxidation were 2-mercaptol ethanol, caffeine, sodium metabisulfite, polyvinylpyrrolidone, cysteine HC1, and ascorbic acid
Main Authors: | , , |
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Format: | biblioteca |
Published: |
1976
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Subjects: | THEOBROMA CACAO, CALLO, ANALISIS DE TEJIDOS, SUSTANCIAS DE CRECIMIENTO VEGETAL, CULTIVO DE CELULAS, TEOBROMINA, CULTIVO DE TEJIDOS, EXPLANTES, |
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Summary: | Optimal cellular proliferation for cacao leaf tissue and fruit explants occurred on modified Murashige and Skoog's medium supplemented with kinetin (2-6 mg/1) in the absence of indole-3-acetic acid (IAA) or with zeatin (0.25 - 1.0 mg/1) and IAA (0.0 - 0.5 mg/1). Theobromine does not seem to be a regulatory molecule in culture explants of cacao but proved to be an effective agent in the control of phenolic oxidation. Other effective chemicals for the control of phenolic oxidation were 2-mercaptol ethanol, caffeine, sodium metabisulfite, polyvinylpyrrolidone, cysteine HC1, and ascorbic acid |
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