Initiation and growth of tissue cultures of Theobroma cacao
Difficulties were experienced in obtaining sterile explant material from which to initiate the callus cultures. The material which provided the most effective source in terms of numbers of explants, levels of contamination and ease of sterilization was from seedling tissue, grown from seeds which had been transported in the intact pod. The seeds were removed asceptically and germinated in sterile flasks on White's medium solidified with agar, then at 3-4 weeks old the seedling was sectioned into root, stem, cotyledon, hypocotyl and apical bud explants. The medium which promoted the most rapid and sustained callus growth was a modified Murashige and Skoog medium. Callus transferred to a liquid medium formed a cell suspension which by selecting for a low level of aggregational a fast growth rate eventually developed these characteristics. There was evidence for clonal differences in the callus based on growth rate data and appearance and related to the origin of the initial explant tissue. Although the callus was exposed to considerable variation in the nutrient medium, except for the occasional appearance of root-like structures, no regular organogenesis was found
| Main Authors: | , |
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| Format: | biblioteca |
| Published: |
1975
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| Subjects: | CULTIVO DE TEJIDOS, THEOBROMA CACAO, CALLO, MEDIO DE CULTIVO, EXPLANTES, ORGANOGENESIS, |
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| Summary: | Difficulties were experienced in obtaining sterile explant material from which to initiate the callus cultures. The material which provided the most effective source in terms of numbers of explants, levels of contamination and ease of sterilization was from seedling tissue, grown from seeds which had been transported in the intact pod. The seeds were removed asceptically and germinated in sterile flasks on White's medium solidified with agar, then at 3-4 weeks old the seedling was sectioned into root, stem, cotyledon, hypocotyl and apical bud explants. The medium which promoted the most rapid and sustained callus growth was a modified Murashige and Skoog medium. Callus transferred to a liquid medium formed a cell suspension which by selecting for a low level of aggregational a fast growth rate eventually developed these characteristics. There was evidence for clonal differences in the callus based on growth rate data and appearance and related to the origin of the initial explant tissue. Although the callus was exposed to considerable variation in the nutrient medium, except for the occasional appearance of root-like structures, no regular organogenesis was found |
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