The indirect fluorescent antibody test for the detection of circulating antibodies in bovine tuberculosis :
Indirect fluorescent antibody (IFA) tests were carried out on a total of 545 individual serums from beef cattle just before tuberculin testing, using specific FITC-anti-bovine IgG and IgM conjugates. Phenol-killed M. tuberculosis (H37Rv strain) or field strains of M. bovis and M. avium were used as antigenic substrates. All cattle were examined at abattoirs for the presence of tuberculous lesions. A statistically significant difference (p minor than 0.001) was found between the number of cattle with serums containing IgM and those with IgG antibody to M. bovis. The majority of cross-reactions that occurred with M. avium was due to IgM antibody. The IFA test using M. bovis and both antiglobulin conjugates detected 77 per cent of cattle with visible lesions of tuberculosis, while the single intradermal caudal fold tuberculin test detected 83 per cent. The IFA test detected 25 of 31 cattle that failed to react in the tuberculin test. The tuberculin test gave apparently false positive reactions in 6.2 per cent and the IFA test gave false positive reactions in 21.2 per cent of all cattle. The IFA test is of potential use as a further aid in the detection of tuberculous cattle that fail to react to tuberculin. It offers no advantage over the tuberculin test for the detection of all forms of the disease because it fails to detect a significant proportion of tuberculous cattle and produces an unacceptably high number of false positive results
| Main Authors: | , |
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| Format: | biblioteca |
| Language: | eng |
| Published: |
May
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| Subjects: | DIAGNOSTICO DE LABORATORIO, PRUEBA TUBERCULINICA, TUBERCULOSIS BOVINA, LESIONES, MYCOBACTERIUM, BACTERIOSIS, ANTICUERPOS, REACCION ANTIGENO-ANTICUERPO, TUBERCULINA, TECNICAS INMUNOLOGICAS, GANADO DE CARNE, |
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| Summary: | Indirect fluorescent antibody (IFA) tests were carried out on a total of 545 individual serums from beef cattle just before tuberculin testing, using specific FITC-anti-bovine IgG and IgM conjugates. Phenol-killed M. tuberculosis (H37Rv strain) or field strains of M. bovis and M. avium were used as antigenic substrates. All cattle were examined at abattoirs for the presence of tuberculous lesions. A statistically significant difference (p minor than 0.001) was found between the number of cattle with serums containing IgM and those with IgG antibody to M. bovis. The majority of cross-reactions that occurred with M. avium was due to IgM antibody. The IFA test using M. bovis and both antiglobulin conjugates detected 77 per cent of cattle with visible lesions of tuberculosis, while the single intradermal caudal fold tuberculin test detected 83 per cent. The IFA test detected 25 of 31 cattle that failed to react in the tuberculin test. The tuberculin test gave apparently false positive reactions in 6.2 per cent and the IFA test gave false positive reactions in 21.2 per cent of all cattle. The IFA test is of potential use as a further aid in the detection of tuberculous cattle that fail to react to tuberculin. It offers no advantage over the tuberculin test for the detection of all forms of the disease because it fails to detect a significant proportion of tuberculous cattle and produces an unacceptably high number of false positive results |
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