Use of PPD and phosphatide antigens in an ELISA to detect the serological response in experimental bovine tuberculosis
Five calves from tuberculosis free herds were each inoculated intranasally with 10 to the sixth power viable organisms of a field isolate of Mycobacterium bovis. Four of the calves developed acute tuberculosis. ELISA's employing protein and phosphatide extracts of M. bovis as antigens were used to monitor the humoral response of the infected calves. Fourteen days after infection there was a dramatic increase in the level of antibodies demonstrated by the phosphatide antigen. This increase coincided with the first appearance of signs of the disease. The results suggest that the phosphatide antigen may be of potential value in detecting a humoral response, if present, in cattle infected with M. bovis. The tests employing the protein antigen demonstrated a humoral response in only one of the infected calves and emphasizes the importance of antigen selection to detect antibodies
| Main Authors: | , , |
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| Format: | biblioteca |
| Published: |
Jul
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| Subjects: | RESPUESTA INMUNOLOGICA, DIAGNOSTICO DE LABORATORIO, ELISA, TECNICAS INMUNOENZIMATICAS, MYCOBACTERIUM, ANTIGENOS, ANTICUERPOS, TERNERO, INOCULACION, REACCION ANTIGENO-ANTICUERPO, TUBERCULOSIS BOVINA, |
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| Summary: | Five calves from tuberculosis free herds were each inoculated intranasally with 10 to the sixth power viable organisms of a field isolate of Mycobacterium bovis. Four of the calves developed acute tuberculosis. ELISA's employing protein and phosphatide extracts of M. bovis as antigens were used to monitor the humoral response of the infected calves. Fourteen days after infection there was a dramatic increase in the level of antibodies demonstrated by the phosphatide antigen. This increase coincided with the first appearance of signs of the disease. The results suggest that the phosphatide antigen may be of potential value in detecting a humoral response, if present, in cattle infected with M. bovis. The tests employing the protein antigen demonstrated a humoral response in only one of the infected calves and emphasizes the importance of antigen selection to detect antibodies |
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