PHB - degrading Streptomyces sp. SSM 5670 isolation, characterization and PHB - accumulation

PHB - degrading Streptomyces sp. SSM 5670 isolation, characterization and PHB - accumulation

New microbial bioprospecting has become an important way to find new polyhydroxyalkanoate [PHA] producers and degraders. Poly [3-hydroxybutyrate] [PHB], the best known member of the PHAs, has received much attention because it can be degraded completely in different environments without forming any toxic products. In this contribution an actinomycete, designated strain SSM 5670, showed the better response for PHB degradation by the clear-zone method. Nevertheless, it produces PHB in low amounts [5.6 percent dry cell weight]. According to the phylogenetic analysis the strain most similar to the PHB-degrading isolate SSM 5670 was Streptomyces omiyaensis NBRC13449. The selected isolate was characterized by its cultural, morphological and physio-biochemical features and deposited in an Argentine culture collection under the name Streptomyces omiyaensis SSM 5670.

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Bibliographic Details
Main Authors: Yashchuk, O., Miyazaki, Silvia Susana, Hermida, E. B.
Format: Texto biblioteca
Language:spa
Subjects:16S RDNA ANALYSIS, ACCUMULATION, ANTIBIOTIC AGENT, ANTIFUNGAL ACTIVITY, ANTIMICROBIAL ACTIVITY, ARABINOSE, ASPERGILLUS NIGER, BACILLUS SUBTILIS, BACTERIUM CULTURE, BACTERIUM ISOLATION, CARBON SOURCE, CASEIN, CHARACTERIZATION, CONTROLLED STUDY, DNA 16S, ENERGY RESOURCE, ESCHERICHIA COLI, FERMENTATION, FRUCTOSE, GALACTOSE, GLUCOSE, HYDROLYSIS, INOSITOL, ISOLATION, KOCURIA, KOCURIA RHIZOPHILA, MANNITOL, MICROBIAL DEGRADATION, MYCELIUM, NITRIC ACID DERIVATIVE, NITRITE, NONHUMAN, NUCLEOTIDE SEQUENCE, PHYLOGENETIC TREE, PHYLOGENY, POLY[3 HYDROXYBUTYRIC ACID], POLY[3-HYDROXYBUTYRATE], RAFFINOSE, RHAMNOSE, SACCHAROMYCES CEREVISIAE, SCREENING, SODIUM CHLORIDE, SPOROGENESIS, STARCH, STREPTOMYCES, STREPTOMYCES NARBONENSIS, STREPTOMYCES OMIYAENSIS, STREPTOMYCES TANASHIENSIS, STREPTOMYCES WEDMORENSIS, STREPTOMYCIN, SUCROSE, SURFACE PROPERTY, XYLOSE,
Online Access:http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=47127
http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=
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Summary:New microbial bioprospecting has become an important way to find new polyhydroxyalkanoate [PHA] producers and degraders. Poly [3-hydroxybutyrate] [PHB], the best known member of the PHAs, has received much attention because it can be degraded completely in different environments without forming any toxic products. In this contribution an actinomycete, designated strain SSM 5670, showed the better response for PHB degradation by the clear-zone method. Nevertheless, it produces PHB in low amounts [5.6 percent dry cell weight]. According to the phylogenetic analysis the strain most similar to the PHB-degrading isolate SSM 5670 was Streptomyces omiyaensis NBRC13449. The selected isolate was characterized by its cultural, morphological and physio-biochemical features and deposited in an Argentine culture collection under the name Streptomyces omiyaensis SSM 5670.

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