Alterations of fibrin networks mediated by sulfated polysaccharides from green seaweeds
Sulfated polysaccharides (SP) extracted from marine green algae are becoming a focus of interest due to their anticoagulant activities [1]. It has been reported that sulfation degree and, consequently electronegative-charge densities, are important structural determinants for the anticoagulant activity, however, other structural features could also influence their anticoagulant effect. Therefore, in order to contribute to the potential development of new antithrombotic agents, the knowledge of specific structural characteristics of these products and their interaction with the different proteins involved in the hemostatic system must be considered. We focused the present work on two different polysaccharides extracted from cell walls of green seaweeds. Fernández et al. [2] reported the isolation and characterization of a highly sulfated linear (1 → 3)-β-L-arabinan, obtained from Codium vermilara (Bryopsidales), Ab1 (Suppl. Fig. 1A), as well as the analysis of its anticoagulant behavior. On the other hand, we have previously reported that a polysaccharide extracted from Penicillus capitatus (Bryopsidales), PF1, a sulfated pyruvylated (1 →3) (1 → 6)-β-Dgalactan (Suppl. Fig. 1B) [3], also exerted anticoagulant activity. It is well known that formation and lysis of the blood clot play an essential role in hemostasis, and changes in these physiological processes can modulate the resulting fibrin network features, rendering a clot more or less thrombogenic [4]. The aim of our study was to evaluate the in vitro effects on formation, structure, and lysis of plasma fibrin networks, given by Ab1 and PF1.
| Main Authors: | , , , , |
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| Format: | Texto biblioteca |
| Language: | eng |
| Subjects: | SULFATED POLYSACCHARIDES, ANTICOAGULANT ACTIVITY, FIBRIN NETWORK STRUCTURE, FIBRINOLYSIS, |
| Online Access: | http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=45488 http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= |
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| Summary: | Sulfated polysaccharides (SP) extracted from marine green algae are becoming a focus of interest due to their anticoagulant activities [1]. It has been reported that sulfation degree and, consequently electronegative-charge densities, are important structural determinants for the anticoagulant activity, however, other structural features could also influence their anticoagulant effect. Therefore, in order to contribute to the potential development of new antithrombotic agents, the knowledge of specific structural characteristics of these products and their interaction with the different proteins involved in the hemostatic system must be considered. We focused the present work on two different polysaccharides extracted from cell walls of green seaweeds. Fernández et al. [2] reported the isolation and characterization of a highly sulfated linear (1 → 3)-β-L-arabinan,
obtained from Codium vermilara (Bryopsidales), Ab1 (Suppl. Fig. 1A), as well as the analysis of its anticoagulant behavior. On the other hand, we have previously reported that a polysaccharide extracted from Penicillus capitatus (Bryopsidales), PF1, a sulfated pyruvylated (1 →3) (1 → 6)-β-Dgalactan (Suppl. Fig. 1B) [3], also exerted anticoagulant activity. It is well known that formation and lysis of the blood clot play an essential role in
hemostasis, and changes in these physiological processes can modulate the resulting fibrin network features, rendering a clot more or less thrombogenic [4]. The aim of our study was to evaluate the in vitro effects on formation, structure, and lysis of plasma fibrin networks, given by Ab1 and PF1. |
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