Allelic frequency of Porcine Stress Syndrome in Uruguay (PCR-RFLP analysis)
Porcine Stress Syndrome (PSS) is a hereditary disease caused by a point mutation (C T) in the gene encoding the ryanodine receptor (CRC1). The PSS generates significant economic losses in the swine industry due to sudden death of animals and / or decrease in the quality of the meat. DNA molecular markers are a safe method for genotype determination of the mutation. In this paper, we study the allele frequency of this mutation by PCR-RFLP in a total of 64 animals of different breeds (Creole pigs Pampa Rocha, hybrids, Landrace, Large White and Duroc). Of these, 48.43% had a homozygous normal genotype (NN), 40.62% had a heterozygous genotype, being mutation carriers (Nn) and the oher 10.93% had a homozygous recessive genotype (nn). This study is the first one in Uruguay where, using molecular techniques, the following groups could be determined in a sample from a population : a. Carriers Nn (Landrace, Large White and commercial hybrids); b. affected animals nn (Landrace and commercial hybrids) and c. Pampa Rocha animals where the n allele was not present.
| Main Authors: | , , , |
|---|---|
| Format: | Digital revista |
| Language: | spa |
| Published: |
Sociedad de Medicina Veterinaria del Uruguay (SMVU)
2010
|
| Online Access: | https://www.revistasmvu.com.uy/index.php/smvu/article/view/172 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|