Detection of antimicrobial residues in muscle, liver and kidney of pork for sale in Bogotá
In this study, the presence of antimicrobials residues in pork tissues of markets, neighborhood groceries and butcher's shops in Bogota city (Colombia) was investigated. The study was performed following a two-step procedure: the first step consisting in the detection of beta-lactams, streptomycin and sulfamethazine traces using the microbiological method of the three plates with Bacillus subtilis; and the second step, following the commercial method ELISA for the samples showing a positive result. The samples were considered positive only if the presence of antimicrobials residues was confirmed by the two methods. The agreement in the results obtained by the two methods was also estimated (k). Out of 212 samples analyzed, 33.0% were positive for at least one antimicrobial product. For liver samples, 45.2% were positive to beta-lactams, 10.8% to streptomycin and 28.6% to sulfamethazine. In the case of kidney 44.8% were positive to penicillin, 10.3% to streptomycin and 23.1% to sulfamethazine. Muscle showed a 9.7% positive result to beta-lactams, 0% to streptomycin and the only sample positive to sulfamethazine by the microbiological method, was also positive by the ELISA method. The results obtained by the two methods (microbiological and ELISA) were in agreement (k > 0.60) and they indicate a high presence of antimicrobials in the commercialized pork tissues. This fact indicates that the slaughter of animals without respecting the time of retirement is a common practice and it shows the need to implement a waste plan throughout the pork meat chain.
| Main Authors: | , |
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| Format: | Digital revista |
| Language: | spa |
| Published: |
Universidad de Ciencias Aplicadas y Ambientales U.D.C.A
2016
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| Online Access: | https://revistas.udca.edu.co/index.php/ruadc/article/view/91 |
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