Antagonism of Pseudomonas fluorescens Migula facing Fusarium oxysporum fsp. pisi Schtdl on pea Pisum sativum L.
The yellowing pea caused by Fusarium oxysporum is the most problem in the crop and its damage to bring economic losses. This research was carried out in the laboratory and greenhouse of the University of Nariño, located at 2486 masl to an average temperature of 13°C., in order to evaluate the antagonism of P. fluorescent facing F. oxysporum on pea Pisum sativum L. The isolation of F. oxysporum affected tissue was made from roots and stems of pea plants, which were multiplied and purified on PDA, strain selection was based on the prevalence in all zones and the speed in their growth, obtaining the strain FTR. The isolation of P. fluorescent was made from roots and stems of healthy pea plants that were in focus with yellowing, which were purified and multiplied in Selective Cetrimide Agar, obtaining strains PPC, PPO and PPL; after biochemical tests. The tests were made in dual in medium Nutrient Agar, in which the strain showed higher PPC antagonism to control the growth of F. oxysporum under in vitro conditions in the laboratory. In greenhouse tests the incidence rose from 90.31% to 6.55% with the bacteria compared with the control, showing the efficiency of the antagonistic strain P. fluorescens PPC in the control of F. oxysporum under controlled conditions.
| Main Authors: | , , |
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| Format: | Digital revista |
| Language: | spa |
| Published: |
Universidad de Ciencias Aplicadas y Ambientales U.D.C.A
2011
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| Online Access: | https://revistas.udca.edu.co/index.php/ruadc/article/view/773 |
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