Response of IFN-y and IL-4 in mice inoculated withrabies virus recombinant G protein
The aim of this study was to compare the performance of IFN- and IL-4 in mice inoculated with recombinant rabiesvirus G protein that was expressed either in transgenic maize or baculovirus. For this purpose, groups of mice wereinoculated as follows: Group 1, inactivated rabies vaccine im; Group 2, plant-derived recombinant G protein orally;Group 3, baculovirus-expressed G protein im; Group 4, baculovirus-expressed G protein orally; and group 5,untransformed maize orally. The levels of IFN- and IL-4 and specific antibodies were evaluated every 15 d. Achallenge was performed at d 60 post-inoculation (pi). Groups 1 and 3 promoted the best humoral response. Onthe other hand, the results showed that the same groups showed the best levels of IFN- at d 10 pi; while thepick of IL4 was detected at d 15 pi. For the survival study, 83 % of the mice immunized with inactivated vaccine,maize or im baculovirus extracts survived viral infection. The recombinant rabies G protein expressed in baculoviruspromoted IFN- and antibodies in a similar way to the inactivated rabies vaccine. In spite of this, mice fed withtransgenic maize survived to the challenge at the same percentage than group 3.
| Main Authors: | , , , , , , |
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| Format: | Digital revista |
| Language: | spa |
| Published: |
Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias
2015
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| Online Access: | https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/4102 |
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