A multiplex PCR assay for detection of genus Salmonella, subspecies I , and serotype Typhimurium in milk
Gastrointestinal disea ses caused by foodstuffs contaminated with Salmonella entérica implicate important social and economic issues, making it necessary to have fast, sensitive, and specific systems for opportune detection of the pathogen. An endpoint PCR protocol for identification of S. entérica in milk is presented using primer pairs previously validated as specific to the genus Salmonella (STM3098-f2/STM3098-r2), subspecies entérica (subspecies I) (STM4057-f/STM4057-r), and serotype Typhimurium (STM4497-f/ STM4497-r). This protocol allowed for detection of 1 CFU/25 mL of spiked milk after a 12 h pre-enrichment period by means of simple and multiplex PCR assay with the three used primer pairs. The designed protocol can be applied in sanita ry survey programs for detection of S. entérica in milk.
| Main Authors: | , , |
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| Format: | Digital revista |
| Language: | spa |
| Published: |
Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias
2015
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| Online Access: | https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/4095 |
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