derivadosVirulence of Listeria monocytogenes strains from goatsand their byproducts
The virulence of 12 Listeria monocytogenes strains from goat and goat by-products was evaluated. Variable virulencelevels were found when compared the relative virulence (mortality, %) and the ability to grow infect the spleen ofBALB/c mice inoculated intravenously or intragastrically. Two strains from feed produced 100 % mortality, by eitherinoculation route and seven strains, including those from feed, infected the spleen with both inoculation routes. Astrain inoculated intravenously without causing death in the mice produced spleen counts similar to those obtainedin dead mice, this could indicate that a high count of the microorganism in the spleen not necessarily ensures itsvirulence. We also tested the capacity to infect J774 macrophages and Caco-2 epithelial cells, finding higher invasionand intracellular growth in Caco-2 with respect the macrophages and showing quadratic growth kinetics in both celllines. Only the two feed strains did not show a significant difference from the control strain (P>0.05) regardingkinetic parameters and invasion percentage in both cell lines. Although all isolates were able to invade Caco-2 cells,not all isolates were able to invade the spleen after the intragastrically inoculation. Two goats feed strains were themost virulent, representing a risk for animal and human health.
| Main Authors: | , , , , |
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| Format: | Digital revista |
| Language: | spa |
| Published: |
Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias
2015
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| Online Access: | https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/3980 |
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