Histophilus somni (Haemophilus somnus) isolated from dairy cattle with reproductive disorders. First report in Mexico
Histophilus somni (Haemophilus somnus) has been recognized as an important pathogen of cattle, associated with reproductive disorders such as vaginitis, endometritis, infertility, and abortion. The purpose of this research was to determine whether H. somni is found in dairy cattle with reproductive tract disease. Convenience sampling was performed in 6 dairies located in central Mexico. Vaginal exudate samples were obtained from 67 cows with reproductive tract disease and from 45 clinically healthy cows. Samples were seeded in chocolate agar then incubated at 37 °C for 24 to 48 h with 10% CO2. Field isolates were characterized using biochemical tests, total protein profile electrophoresis, immunotransference (Western blot), and PCR. An ATCC reference strain was used at all times as a positive control. From a total of 112 animals sampled, 10 (8.9%) H. somni isolates were obtained. Isolates were recovered only from cows with reproductive tract disease, representing 14 % (10/67) within this group. The organism was not isolated from any of the 45 clinically healthy cows. This is the first report of the presence of H. somni in dairy cattle with reproductive disorders in Mexico
| Main Authors: | , , , , |
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| Format: | Digital revista |
| Language: | spa |
| Published: |
Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias
2012
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| Online Access: | https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/1381 |
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