Bacteriological and serological study of brucellosis in cows revaccinated with reduced doses of Brucella abortus strain 19
Bustamante SJ, Salazar HFI, Díaz AE, Manzano CC, Pérez GR, Hernández AL. Téc Pecu Méx. 2000;38(1)35-42. The goals were to determine by different diagnostic tests, wether the antibodies in cows are due to infection or revaccination with strain 19 Brucella abortus reduced dose; and also to know the biotypes and strains isolated. One hundred Holstein cows from 25 herds in the state of Hidalgo were vaccinated when three to six months old with strain 19 classic dose (1x1010 cfu/ ml) and revaccinated when they were adult. One hundred samples of serum and vaginal exudate and 90 milk samples were taken. The complement fixation, rivanol and radial immunodifusion (RID) with native haptene tests were made. The isolation of Brucella was done according to techniques previously reported. After typing, ten isolated strains were identified as B. abortus biotype 1; 52% of the cows were positive to complement fixation test, 87% to rivanol and 74% for RID. Only RID positive animals were considered true positives; 26% of animals positive to card test and negative to RID were considered false positive due to revaccination antibodies. The official tests were not good enough to differentiate animals revaccinated with strain 19. From infected animals, RID was the best test for diagnostic purposes.
| Main Authors: | , , , , , |
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| Format: | Digital revista |
| Language: | spa |
| Published: |
Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias
2012
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| Online Access: | https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/1354 |
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