Identification of zygotic trifoliate orange seedlings with RAPD molecular markers
This work aimed to determine germination frequency of zygotic embryos of seeds originated from open pollinated trifoliate orange [Poncirus trifoliata (L.) Raf.], using RAPD molecular markers. Leaves and seeds of P. trifoliata were collected from five commercial nurseries and from a citrus germplasm collection. The seeds were placed to germinate in plastic tubes in a greenhouse, reaching a final sample of 62 seedlings. Nine primers were used and the band patterns generated from seedlings leaves were compared to progenitor-plant-leaf hands. It was possible to identify 3.23% of seedlings originated from zygotic embryos which is acceptable in propagation by seeds in this rootstock.
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| Main Authors: | , , |
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| Format: | Digital revista |
| Language: | por |
| Published: |
Pesquisa Agropecuaria Brasileira
2004
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| Online Access: | https://seer.sct.embrapa.br/index.php/pab/article/view/6752 |
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