Production of recombinant glycoprotein C from Equid alphaherpesvirus 1 and evaluation of the immune response induced in mice
Equid alfaherpesvirus 1 (EHV-1) causes respiratory disease, abortions, perinatal death and neurological disorders in horses. Envelope glycoproteins are the most immunodominant antigens that generate an immune response in infected animals. Natural infection and available vaccines provide partial and short-term protection against reinfection. In this study, EHV-1 glycoprotein C was expressed with a recombinant baculovirus and the ability to induce protective immunity in BALB / c mice when challenged with the virus was analyzed. Different routes of administration and adjuvants were evaluated. Glycoprotein C induced a specific IgG response in serum, however, it failed to prevent the entry of the virus into the lung or the appearance of clinical signs, which were variable among different groups of mice. In all immunized groups the entry of the virus into the lung only was delayed. When adjuvants were used, the viral titer was significantly lower than in the control group, as well as in the intranasally immunized group. Therefore, the glycoprotein C induced partial protection against the challenge with the virus depending on the adjuvant and the route of immunization used.
| Main Authors: | , , , , , , , |
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| Format: | Digital revista |
| Language: | spa |
| Published: |
Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata
2021
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| Online Access: | https://revistas.unlp.edu.ar/analecta/article/view/10909 |
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