Immunophenotype of lung granulomas in HIV and non-HIV associated tuberculosis

OBJECTIVE: To describe the immunophenotype of pulmonary TB granulomas from autopsied patients with tuberculosis (TB group) and from HIV patients with tuberculosis (TB/HIV group), and to identify the Mycobacterium species using polymerase chain reaction (PCR) technique. METHODS: Lung samples of 15 TB group patients and 23 TB/HIV group patients were selected. Histopathologic analyses and immunohistochemistry tests were performed to describe the granulomas and to detect the infectious agent (anti-BCG). CD4, CD8, CD20 and CD68 were evaluated to characterize the immnophenotype of the granulomas. Polymerase Chain Reaction was performed to identify the mycobacterium species. RESULTS: CD4 + T lymphocytes were the cells with highest density in the TB group, whereas CD68 cells exhibited the highest density in the TB/HIV group. Comparison between groups showed that the CD4 + T density was significantly higher in the TB patients; whereas, CD68 density was significantly higher in the TB/HIV patients. M. tuberculosis was identified in 8 cases of each group; M. avium was only found in one case of the TB/HIV group. CONCLUSION: With the advent of AIDS, the immunological profile of TB has changed. This may be associated with the depletion of CD4 + T lymphocytes in lung granulomas. M. tuberculosis was the major etiological agent of TB in both groups.

Bibliographic Details

Main Authors:	Silva,Andreia A., Mauad,Thais, Saldiva,Paulo H.N., Pires-Neto,Ruy C., Coletta,Ricardo D., Graner,Edgard, Vargas,Pablo A.
Format:	Digital revista
Language:	English
Published:	Mavera Edições Técnicas e Científicas Ltda 2014
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2358-04292014000400174
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