Host and Vectors of Xylella fastidiosa in Parras, Coahuila Vineyards, Mexico
Abstract: Xylella fastidiosa is a phytopathogenic bacterium that causes important diseases in different crops such as Pierce’s disease in grapevine (Vitis vinifera), citrus variegated chlorosis, among others. The bacterium spreads very fast through vector insects, mainly leafhoppers (Cicadellidae) and spittlebugs (Cercopidae). The objective of present investigation was to determine the vectors and hosts of Xylella fastidiosa in vineyards located in Parras valley, Coahuila. Samples of 22 species were collected, including ornamental plants, weeds, shrubs, fruit trees, evergreen and deciduous forest; At the same time, the insects found in the sampling areas were collected and identified. The detection of X. fastidiosa in plants and insects was performed by PCR using the primers RST31/RST33. In the same way, identification of X. fastidiosa subsp. Multiplex with primers ALM1/ALM2 and XF2542-L/XF2542-R in plant samples that were positive in first test was carried out. The presence of X. fastidiosa was detected in commercial vine and wild grape plants, while X. fastidiosa subsp. multiplex was identified in apricot and ash trees. Among the insects collected, six genera and four species of leafhoppers were identified, however, only X. fastidiosa was detected in Homalodisca vitripennis.
| Main Authors: | , , , , , |
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| Format: | Digital revista |
| Language: | English |
| Published: |
Universidad Autónoma de Nayarit
2019
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| Online Access: | http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S2007-33802019000100130 |
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