Validation of a UV-spectrophotometric analytical method for determination of LPSF/AC04 from inclusion complex and liposomes

The aim of this study was to develop and validate a UV spectrophotometric method for determination of LPSF/AC04 from inclusion complex and encapsulated into liposomes. The validation parameters were determined according to the International Conference on Harmonisation (ICH) and National Health Surveillance Agency (ANVISA) guidelines. LPSF/AC04 was determined at 250 nm in methanol by a UV spectrophotometric method, exhibiting linearity in the range from 0.3 to 2 µg.mL−1 (Absorbance=0.18068 x [LPSF/AC04 µg.mL-1] + 0.00348), (r2=0.9995). The limits of detection and quantification were 0.047µg.mL−1 and 0.143µg.mL−1, respectively. The method was accurate, precise, reproducible and robust since all the samples analyzed had coefficient of variation of less than 5% and no statistically significant difference between theoretical and practical concentrations was detected. Thus, a rapid, simple, low cost and sensitive spectrophotometric method was developed and validated for determining the content of inclusion complex and liposomes containing LPSF/AC04.

Bibliographic Details

Main Authors:	Ferraz,Rafaela Siqueira, Mendonça,Elisângela Afonso Moura, Silva,Jéssica Priscila Avelino, Cavalcanti,Isabella Macário Ferro, Lira-Nogueira,Mariane Cajubá Britto, Galdino,Suely Lins, Pitta,Ivan Rocha, Lima,Maria do Carmo Alves, Santos-Magalhães,Nereide Stela
Format:	Digital revista
Language:	English
Published:	Universidade de São Paulo, Faculdade de Ciências Farmacêuticas 2015
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000100183
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