Total polyphenols from Syzygium cumini (L.) Skeels fruit extract

A precise, accurate and low cost spectrophotometric method was developed and validated for routine determination of total polyphenols, as pyrogallic acid equivalents, from the percolated and lyophilized extract of Syzygium cumini (L.) Skeels fruits. Validation was assessed experimentally and data were rigorously treated by statistical analysis. Analytical parameters were: linearity, interval (range), precision and recovery/accuracy, limit of detection (LOD, μg mL-1) and limit of quantification (LOQ, μg mL-1). The visible spectrophotometric method presented linearity (r² = 0.9979 ± 0.0010) over the concentration range 0.25-7.5 μg mL-1 of standard pyrogallic acid, precision < 2.918171%, recovery/accuracy ranging from 96.228693 to 107.17701%, LOD = 0.21 μg mL-1 and LOQ = 0.64 μg mL-1.

Bibliographic Details

Main Authors:	Migliato,Ketylin Fernanda, Carvalho,Elisângela Simões de, Sacramento,Luis Vitor Silva do, Mello,João Carlos Palazzo de, Baby,André Rolim, Velasco,Maria Valéria Robles, Salgado,Hérida Regina Nunes
Format:	Digital revista
Language:	English
Published:	Universidade de São Paulo, Faculdade de Ciências Farmacêuticas 2009
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000100015
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