Chemical constituents of Calotropis procera latex and ultrastructural effects on Haemonchus contortus

Abstract This study aimed to evaluate the anthelmintic and ultrastructural effects of Calotropis procera latex on Haemonchus contortus. C. procera latex was twice centrifuged at 10,000×g and dialyzed to obtain a fraction rich in proteins, named LP (latex protein), and at 3,000 rpm to obtain a fraction rich in secondary metabolites, named LNP (latex non-protein). Specimens of H. contortus exposed to LNP, LP and PBS in the Adult Worm Motility Test (AWMT) were submitted to scanning (SEM) and transmission (TEM) electron microscopy to verify changes in their ultrastructure. Phytochemical tests in the LNP indicated the presence of phenols, steroids, alkaloids and cardenolides. High-Performance Liquid Chromatography (HPLC) characterized the presence of the compounds gallic acid and quercetin in the LNP. The protein content in the LP was 43.1 ± 1.1 mg/mL and 7.7 ± 0.3 mg/mL in LNP. In AWMT, LNP and LP inhibited the motility of 100% of the nematodes, with LNP being more effective than LP and ivermectin more effective than both (p <0.05). Cuticle changes were observed by SEM and TEM in nematodes treated with LP and LNP. Calotropis procera latex has anthelmintic effects against H. contortus, causing damage to its cuticle and other alterations in its ultrastructure.

Bibliographic Details

Main Authors:	Cavalcante,Géssica Soares, Morais,Selene Maia de, André,Weibson Paz Pinheiro, Araújo-Filho,José Vilemar de, Muniz,Celli Rodrigues, Rocha,Letícia Oliveira da, Ribeiro,Wesley Lyeverton Correia, Rodrigues,Ana Livya Moreira, Oliveira,Lorena Mayana Beserra de, Bevilaqua,Claudia Maria Leal, Ramos,Márcio Viana
Format:	Digital revista
Language:	English
Published:	Colégio Brasileiro de Parasitologia Veterinária 2020
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612020000200324
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