Expression of Matrix Metalloproteinases-8 and Myeloperoxidase in Pulp Tissue after Pulpotomy with Calcium Silicate Cements

ABSTRACT Objective: To evaluate the pulp tissue of rat molars after pulpotomies with mineral trioxide aggregate (MTA), BiodentineTM (BDT) and calcium hydroxide (CH) mixed with sterile saline solution (24 hours, 72 hours, 7 days and 15 days), through correlating MPO activity with active neutrophils and MMP8 activity with tissue remodeling. Material and Methods: Thirty-eight Wistar rats were randomly distributed into groups (control, I (MTA gray), II (BDT), and III (CH)) and subdivided according to the study period of 24 hours, 72 hours, 7 days or 15 days after pulpotomy. MMP8 activity was assessed through fluorescence technique, and MPO activity was determined using the MPO assay. Results: A gradual decrease of MPO and MM8 activity occurred in the group MTA over the experimental periods (p<0.05). Groups BDT and CH exhibited an increase in the activity at 7 and 15 days (p<0.05). Conclusion: MTA demonstrated a decrease in the values of MPO e MMP8. BDT and CH showed high neutrophilic and collagenase activity over the experimental periods.

Bibliographic Details

Main Authors:	Cunha,Nayara Nery de Oliveira, Junqueira,Marina Azevedo, Cosme-Silva,Leopoldo, Santos,Laís da Silveira Terra, Oliveira,George Augusto Veloso de, Moretti Neto,Rafael Tobias, Nogueira,Denismar Alves, Brigagão,Maísa Ribeiro Pereira Lima, Moretti,Ana Beatriz da Silveira
Format:	Digital revista
Language:	English
Published:	Associação de Apoio à Pesquisa em Saúde Bucal 2021
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1983-46322021000100322
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