Immobilization of Thermomyces lanuginosus Lipase in PVA-alginate Beads

Thermomyces lanuginosus lipase was immobilized in PVA-alginate beads, obtaining immobilization % in the range of 94.4-98.4% using PVA concentrations ranging from 11% to 12.5%, and with cross linking times of 45 and 60 min using boric acid. Initial reaction rate was determined in free and immobilized state by hydrolysis of p-nitrophenol palmitate. Operational stability at different pH (4-7), agitation (100-500 r.p.m.), and temperature (40-80 °C) was investigated. Results showed that pH 6 and 7 no considerable loss of enzyme activity or enzyme was observed. At temperatures over 70 °C, enzyme suffers physical damage and showed a considerable loss of activity. No significant difference was observed when agitation was varied from 100 to 500 r.p.m.

Bibliographic Details

Main Authors:	Cruz-Ortiz,Brenda Rogelina, Ríos-González,Leopoldo Javier, Garza García,Yolanda, Rodríguez de la Garza,José Antonio, Rodríguez-Martínez,Jesús
Format:	Digital revista
Language:	English
Published:	Sociedad Química de México A.C. 2011
Online Access:	http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1870-249X2011000300008
Tags:	Add Tag No Tags, Be the first to tag this record!