THE ACTION OF SERICIN PROTEIN ON INITIAL NERVE REPAIR, ASSOCIATED OR NOT WITH SWIMMING IN WISTAR RATS

ABSTRACT Objective: To analyze the effects of sericin treatment, associated or not with swimming with load exercise, on initial sciatic nerve repair after compression in Wistar rats. Methods: Forty animals were divided into five groups: control, injury, injury-sericin, injury-swimming and injury-sericin-swimming. During the axonotmesis procedure, the sericin was applied to the injury-sericin and injury-sericin-swimming groups. The injury-swimming and injury-sericin-swimming groups performed the swimming with load exercise for five days, beginning on the third postoperative day (PO), and were evaluated for function, nociception and allodynia. Euthanasia was performed on the 8th PO day and fragments of the nerve were collected and prepared for quantitative and descriptive analysis in relation to the total amount of viable nerve fibers and non-viable nerve fibers, nerve fiber diameter, axon diameter and myelin sheath thickness. Results: There was no significant improvement in the sciatic functional index up to the eighth day. The Von Frey test of the surgical scar and plantar fascia indicated a reduction in pain and allodynia for the injury-swimming and injury-sericin-swimming groups. The morphological analysis presented similar characteristics in the injury-sericin, injury-swimming and injury-sericin-swimming groups, but there was a significant difference in the number of smaller non-viable nerve fibers in the injury-swimming and injury-sericin-swimming groups as compared to the others. Conclusions: Isolated sericin protein presented proinflammatory characteristics. There was improvement of allodynia and a decrease in the pain at the site of the surgical incision, possibly linked to an aquatic effect. There was no acceleration of nerve repair on the eighth day after the injury. Level of Evidence I; High quality randomized clinical trial with or without statistically significant difference, but with narrow confidence intervals.

Bibliographic Details

Main Authors:	Debastiani,Jean Carlos, Santana Junior,André, Tavares,Alana Ludemila de Freitas, Costa,Juliana Roncini Gomes da, Machado,Luiz Gustavo Vasconcelos, Kunz,Regina Inês, Ribeiro,Lucinéia de Fátima Chasko, Costa,Rose Meire, Bertolini,Gladson Ricardo Flor
Format:	Digital revista
Language:	English
Published:	Sociedade Brasileira de Coluna 2019
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-18512019000400262
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