Usefulness of aqueous and vitreous humor analysis in infectious uveitis

OBJECTIVE: To evaluate the role of intraocular fluid analysis as a diagnostic aid for uveitis. METHODS: Twenty-eight samples (27 patients including 3 HIV-infected patients) with active (n=24) or non-active (n=4) uveitis were submitted to aqueous (AH; n=12) or vitreous humor (VH) analysis (n=16). All samples were analyzed by quantitative PCR for herpes simplex virus (HSV), varicella zoster virus (VZV), cytomegalovirus (CMV), Epstein-Barr virus (EBV) and Toxoplasma gondii. RESULTS: The positivity of the PCR in AH was 41.7% (5/12), with 50% (2/4) in immunocompetent and 67% (2/3) in HIV+ patients. The positivity of the PCR in VH was 31.2% (5/16), with 13% (1/8) in immunocompetent and 50% (4/8) in immunosuppressed HIV negative patients. The analysis was a determinant in the diagnostic definition in 58% of HA and 50% of VH. CONCLUSION: Even in posterior uveitis, initial AH analysis may be helpful. A careful formulation of possible clinical diagnosis seems to increase the chance of intraocular sample analysis being meaningful.

Bibliographic Details

Main Authors:	Santos,Helen Nazareth Veloso dos, Ferracioli-Oda,Eduardo, Barbosa,Thaisa Silveira, Otani,Camila Sayuri Vicentini, Tanaka,Tatiana, Silva,Luciane de Carvalho Sarahyba da, Lopes,Guilherme de Oliveira, Doi,Andre, Hirata,Carlos Eduardo, Yamamoto,Joyce Hisae
Format:	Digital revista
Language:	English
Published:	Faculdade de Medicina / USP 2020
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322020000100210
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