Histological study of fresh versus frozen semitendinous muscle tendon allografts

OBJECTIVE: The purpose of this study was to histologically analyze allografts from cadaveric semitendinous muscle after cryopreservation at -80°C in comparison to a control group kept at only -4°C to test the hypothesis that the histological characteristics of the tissue are maintained when the tendons are kept at lower temperatures. METHODS: In a tissue bank, 10 semitendinous tendons from 10 cadavers were frozen at -80ºC as a storage method for tissue preservation. They were kept frozen for 40 days, and then a histological study was carried out. Another 10 tendon samples were analyzed while still "fresh". RESULTS: There was no histological difference between the fresh and frozen samples in relation to seven variables. CONCLUSIONS: Semitendinous muscle tendon allografts can be submitted to cryopreservation at -80ºC without suffering histological modifications.

Bibliographic Details

Main Authors:	Bitar,Alexandre Carneiro, Santos,Luiz Augusto Ubirajara, Croci,Alberto Tesconi, Pereira,João Alberto Ramos Maradei, França Bisneto,Edgard N., Giovani,Arlete Mazzini Miranda, Oliveira,Claudia Regina G. C. M.
Format:	Digital revista
Language:	English
Published:	Faculdade de Medicina / USP 2010
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322010000300010
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