A new experimental model of cigarette smoke-induced emphysema in Wistar rats

OBJECTIVE: To describe a new murine model of cigarette smoke-induced emphysema. METHODS: Twenty-four male Wistar rats were divided into two groups: the cigarette smoke group, comprising 12 rats exposed to smoke from 12 commercial filter cigarettes three times a day (a total of 36 cigarettes per day) every day for 30 weeks; and the control group, comprising 12 rats exposed to room air three times a day every day for 30 weeks. Lung function was assessed by mechanical ventilation, and emphysema was morphometrically assessed by measurement of the mean linear intercept (Lm). RESULTS: The mean weight gain was significantly (approximately ten times) lower in the cigarette smoke group than in the control group. The Lm was 25.0% higher in the cigarette smoke group. There was a trend toward worsening of lung function parameters in the cigarette smoke group. CONCLUSIONS: The new murine model of cigarette smoke-induced emphysema and the methodology employed in the present study are effective and reproducible, representing a promising and economically viable option for use in studies investigating the pathophysiology of and therapeutic approaches to COPD.

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Bibliographic Details
Main Authors: Kozma,Rodrigo de las Heras, Alves,Edson Marcelino, Barbosa-de-Oliveira,Valter Abraao, Lopes,Fernanda Degobbi Tenorio Quirino dos Santos, Guardia,Renan Cenize, Buzo,Henrique Vivi, Faria,Carolina Arruda de, Yamashita,Camila, Cavazzana Junior,Manzelio, Frei,Fernando, Ribeiro-Paes,Maria Jose de Oliveira, Ribeiro-Paes,Joao Tadeu
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Pneumologia e Tisiologia 2014
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-37132014000100046
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