Metformin synergistically enhances antiproliferative effects of cisplatin and etoposide in NCI-H460 human lung cancer cells

OBJECTIVE: To test the effectiveness of combining conventional antineoplastic drugs (cisplatin and etoposide) with metformin in the treatment of non-small cell lung cancer in the NCI-H460 cell line, in order to develop new therapeutic options with high efficacy and low toxicity.METHODS: We used the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and calculated the combination index for the drugs studied.RESULTS: We found that the use of metformin as monotherapy reduced the metabolic viability of the cell line studied. Combining metformin with cisplatin or etoposide produced a synergistic effect and was more effective than was the use of cisplatin or etoposide as monotherapy.CONCLUSIONS: Metformin, due to its independent effects on liver kinase B1, had antiproliferative effects on the NCI-H460 cell line. When metformin was combined with cisplatin or etoposide, the cell death rate was even higher.

Bibliographic Details

Main Authors:	Teixeira,Sarah Fernandes, Guimarães,Isabella dos Santos, Madeira,Klesia Pirola, Daltoé,Renata Dalmaschio, Silva,Ian Victor, Rangel,Leticia Batista Azevedo
Format:	Digital revista
Language:	English
Published:	Sociedade Brasileira de Pneumologia e Tisiologia 2013
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-37132013000600644
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