Physico-chemical characterization of collagen scaffolds for tissue engineering
The objective was to research the physical and chemical properties of collagen scaffolds (CS) obtained from bone matrix Nukbone(r) subject to a demineralization process using hydrochloric acid. The CS samples were characterized by optical and scanning electron microscopy, elemental chemical analysis, X-ray diffraction, spectroscopy Infrared, thermal analysis, differential scanning calorimetry and nitrogen adsorption. The microanalysis were used to set the macro- and microstructures of CS. They showed that the CS retained the morphology of Nukbone(r) with interconnected pores and their size between 100 and 500 μm, and it is composed of 20% by weight of HA and the rest is collagen type I. By infrared spectroscopy the functional groups of collagen type I (amide A - 3285, B - 2917, I - 1633, II - 1553 and III - 1239 cm−1) were identified. By thermal analysis it was determined that the phenomenon of denaturation of the collagen type I began in the range of 75-85 °C and burned above 200 °C.
| Main Authors: | , , , |
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| Format: | Digital revista |
| Language: | English |
| Published: |
Universidad Nacional Autónoma de México, Instituto de Ciencias Aplicadas y Tecnología
2016
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| Online Access: | http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1665-64232016000100077 |
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