In vivo enzyme activity and induction of DNA damage in Swiss albino male mice by automobile waste leachate

Abstract The rapid growth of motor vehicles use, together with poor waste disposal, produce environmental and biological threats. We evaluated the genotoxicity and enzyme activity of simulated automobile waste leachate in Swiss albino male mice (Mus musculus). Four mice per group were intraperitoneally treated with four leachate concentrations (10%, 15%, 20% and 25% v/v: simulant (IOASL)/distilled water), as well as a negative control (0.5mL UILSL); and a positive control (cyclophosphamide 20mg/Kg body weight) for five consecutive days. There was a concentration-dependent increase in sperm abnormality compared to the negative control (except at 10% and 15%; p<0,05). Heavy metal (Pb, Cd, As, Hg, Cr, Cu, Fe and Zn) exceeded permissible limits for waste water. Significant variability was also recorded in liver serum enzyme activity (AST, ALP, ALT and ALB) and in the frequencies of micronuclei (p<0,05). The interaction of some of these components with the genetic constitution of the cell during spermatogenesis might be responsible for the abnormalities.

Bibliographic Details

Main Authors:	Anifowoshe,Abass Toba, Iyiola,Oluyinka Ajibola, Olafimihan,Temitope Fatima, Oladipo,Segun Olayinka, Yakubu,Sunday Frank, Abubakar,Thuwaibat Oyiyoza, Bakare,Rukayat Ifedolapo, Akinseye,Kehinde Monica, Sulaiman,Faoziyat Adenike
Format:	Digital revista
Language:	English
Published:	Universidad Estatal a Distancia de Costa Rica 2018
Online Access:	http://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S1659-42662018000200285
Tags:	Add Tag No Tags, Be the first to tag this record!