Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping

Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping

We report a comparative study of two DNA extraction techniques, thermolysis and chemical lysis (CTAB), for molecular identification and genotyping of M. tuberculosis. Forty DNA samples were subjected to PCR and the results demonstrated that with thermolysis it is possible to obtain useful data that enables fast identification and genotyping.

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Bibliographic Details
Main Authors: Miyata,Marcelo, Santos,Adolfo Carlos Barreto, Mendes,Natália Helena, Cunha,Eunice Atsuko, Melo,Fernando Augusto Fiúza de, Leite,Clarice Queico Fujimura
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Microbiologia 2011
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200045
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