Differentiation of Bovine coronavirus (BCoV) genotypes by a restriction enzyme assay

Differentiation of Bovine coronavirus (BCoV) genotypes by a restriction enzyme assay

This article reports the use of the GsuI restriction enzyme to differentiate genotypes of Bovine Coronavirus (BCoV), based on an 18-nucleotide deletion of S1-coding region found in one of the two genotypes. It was concluded that this assay can be used as a rapid tool for BCoV genotypes differentiation.

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Bibliographic Details
Main Authors: Souza,Sibele Pinheiro de, Asano,Karen Miyuki, Sandri,Thaisa Lucas, Barros,Iracema Nunes de, Richtzenhain,Leonardo José, Brandão,Paulo Eduardo
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Microbiologia 2010
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300034
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