Isolation and characterization of BliAI, an isoschizomer of ClaI from Bacillus licheniformis
The restriction endonuclease BliAI, an isoschizomer of ClaI, which recognizes the sequence 5'- AT<FONT FACE=Symbol>¯</FONT>CGAT - 3', was purified from a natural isolate identified as Bacillus licheniformis. The restriction endonuclease was isolated from cell extracts using single-step purification by phosphocellulose column chromatography. The restriction endonuclease is active at 37ºC and over a wide range of pH and salt concentration. The molecular weight of the purified restriction enzyme is consistent with a value of 39 kDa.
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| Main Authors: | , , , , |
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| Format: | Digital revista |
| Language: | English |
| Published: |
Sociedade Brasileira de Microbiologia
2006
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| Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400027 |
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