Production of pectin lyase by Penicillium griseoroseum in bioreactors in the absence of inducer

Penicillium griseoroseum was grown in bioreactors on mineral medium supplemented with yeast extract and sucrose. The influence of inoculum and carbon source concentrations, aeration and pH on pectin lyase (PL) production, as well as the capacity of P. griseoroseum to produce PL when grown on sugar cane syrup as carbon source were evaluated. Inoculum concentration did not influence PL production. Production was higher in non-aerated than in aerated medium. The best results were obtained using 60 mM sucrose at pH 6.3-7.2. Production using cane syrup 25% (v/v), without yeast extract supplement, was equal to that obtained under the conditions cited above.

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Bibliographic Details
Main Authors: Piccoli-Valle,Roberta Hilsdorf, Passos,Flávia Maria Lopes, Passos,Frederico José Vieira, Silva,Daison Olzany
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Microbiologia 2001
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200013
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