Method for immobilizing invertase by adsorption on Dowex® anionic exchange resin

Method for immobilizing invertase by adsorption on Dowex® anionic exchange resin

This communication describes a method for adsorbing the invertase (EC.3.2.1.26) on DOWEX® anion exchange resin. Among the types of DOWEX® resins studied (1x8:50-400; 1x4:50-400 and 1x2:100-400), 1X4-200 was the most suitable, because it adsorbed the invertase molecules completely and the complex 1X4-200/invertase retained 100% of the catalytic activity. Moreover, no leakage of enzyme from the support was noted at the end of the sucrose hydrolysis.

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Main Authors: Tomotani,Ester Junko, Vitolo,Michele
Format: Digital revista
Language:English
Published: Divisão de Biblioteca e Documentação do Conjunto das Químicas da Universidade de São Paulo 2006
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-93322006000200009
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spelling oai:scielo:S1516-933220060002000092006-08-28Method for immobilizing invertase by adsorption on Dowex® anionic exchange resinTomotani,Ester JunkoVitolo,Michele Invertase Immobilization Anionic exchange resins Adsorption This communication describes a method for adsorbing the invertase (EC.3.2.1.26) on DOWEX® anion exchange resin. Among the types of DOWEX® resins studied (1x8:50-400; 1x4:50-400 and 1x2:100-400), 1X4-200 was the most suitable, because it adsorbed the invertase molecules completely and the complex 1X4-200/invertase retained 100% of the catalytic activity. Moreover, no leakage of enzyme from the support was noted at the end of the sucrose hydrolysis.info:eu-repo/semantics/openAccessDivisão de Biblioteca e Documentação do Conjunto das Químicas da Universidade de São PauloRevista Brasileira de Ciências Farmacêuticas v.42 n.2 20062006-06-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-93322006000200009en10.1590/S1516-93322006000200009
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Tomotani,Ester Junko
Vitolo,Michele
spellingShingle Tomotani,Ester Junko
Vitolo,Michele
Method for immobilizing invertase by adsorption on Dowex® anionic exchange resin
author_facet Tomotani,Ester Junko
Vitolo,Michele
author_sort Tomotani,Ester Junko
title Method for immobilizing invertase by adsorption on Dowex® anionic exchange resin
title_short Method for immobilizing invertase by adsorption on Dowex® anionic exchange resin
title_full Method for immobilizing invertase by adsorption on Dowex® anionic exchange resin
title_fullStr Method for immobilizing invertase by adsorption on Dowex® anionic exchange resin
title_full_unstemmed Method for immobilizing invertase by adsorption on Dowex® anionic exchange resin
title_sort method for immobilizing invertase by adsorption on dowex® anionic exchange resin
description This communication describes a method for adsorbing the invertase (EC.3.2.1.26) on DOWEX® anion exchange resin. Among the types of DOWEX® resins studied (1x8:50-400; 1x4:50-400 and 1x2:100-400), 1X4-200 was the most suitable, because it adsorbed the invertase molecules completely and the complex 1X4-200/invertase retained 100% of the catalytic activity. Moreover, no leakage of enzyme from the support was noted at the end of the sucrose hydrolysis.
publisher Divisão de Biblioteca e Documentação do Conjunto das Químicas da Universidade de São Paulo
publishDate 2006
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-93322006000200009
work_keys_str_mv AT tomotaniesterjunko methodforimmobilizinginvertasebyadsorptionondowexanionicexchangeresin
AT vitolomichele methodforimmobilizinginvertasebyadsorptionondowexanionicexchangeresin
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