Method for immobilizing invertase by adsorption on Dowex® anionic exchange resin
This communication describes a method for adsorbing the invertase (EC.3.2.1.26) on DOWEX® anion exchange resin. Among the types of DOWEX® resins studied (1x8:50-400; 1x4:50-400 and 1x2:100-400), 1X4-200 was the most suitable, because it adsorbed the invertase molecules completely and the complex 1X4-200/invertase retained 100% of the catalytic activity. Moreover, no leakage of enzyme from the support was noted at the end of the sucrose hydrolysis.
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| Main Authors: | , |
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| Format: | Digital revista |
| Language: | English |
| Published: |
Divisão de Biblioteca e Documentação do Conjunto das Químicas da Universidade de São Paulo
2006
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| Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-93322006000200009 |
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