The constitutive expression of the V gene of Parainfluenza virus 5 affects the growth properties of bovine herpesvirus 5

This study aimed to analyze the effect of the expression of Parainfluenza virus 5 (PIV5) V protein in bovine cells on the replication of Bovine herpesvirus 5 (BoHV-5). Growth properties of BoHV-5 were evaluated in parental and PIV5 transfected cells. In one-step growth experiments, the BoHV-5 reached higher titers at earlier time points in the transfected cells when compared to the parental cells. The mean plaque size produced by the BoHV-5 in transfected cells was larger than the parental cells. This indicated that the expression of the PIV5 V gene facilitated the release and cell-to-cell spread of BoHV-5 in bovine cells.

Bibliographic Details

Main Authors:	Lima,Francisco Esmaile de Sales, Batista,Helena Beatriz Ruthner, Campos,Fabrício Souza, Kunert Filho,Hiran Castagnino, Cibulski,Samuel, Finoketti,Fernando, Arantes,Thalita, Spilki,Fernando, Roehe,Paulo Michel, Rijsewijk,Franciscus Antonius Maria, Franco,Ana Cláudia
Format:	Digital revista
Language:	English
Published:	Instituto de Tecnologia do Paraná - Tecpar 2014
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100007
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