Purification of plasmid (pVaxLacZ) by hydrophobic interaction chromatography

Purification of plasmid (pVaxLacZ) by hydrophobic interaction chromatography

This paper describes a method for the plasmid DNA purification, which includes an ammonium sulphate precipitation, followed by hydrophobic interaction chromatography (HIC) using Phenyl Sepharose 6 Fast Flow (low sub). The use of HIC took advantage of the more hydrophobic character of single stranded nucleic acid impurities as compared with double-stranded plasmid DNA.

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Bibliographic Details
Main Authors: Moreira,Keila Aparecida, Diogo,Margarida, Prazeres,Duarte Miguel, Lima Filho,José Luiz de, Porto,Ana Lúcia Figueiredo
Format: Digital revista
Language:English
Published: Instituto de Tecnologia do Paraná - Tecpar 2005
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400014
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