Partial purification and properties of cellulase-free alkaline xylanase produced by Rhizopus stolonifer in solid-state fermentation
Rhizopus stolonifer was cultivated in wheat bran to produce a cellulase-free alkaline xylanase. The purified enzyme obtained after molecular exclusion chromatography in Sephacryl S-200 HR showed optimum temperature as 45º C and hydrolysis pHs optima as pH 6.0 and 9.0. Xylanase presented higher Vmax at pH 9.0 (0.87 µmol/mg protein) than at pH 6.0 and minor Km at pH 6.0 (7.42 mg/mL) than at pH 9.0.
Saved in:
Main Authors: | , , |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Instituto de Tecnologia do Paraná - Tecpar
2005
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300001 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|